ZetaView® Evolution tracks thousands of individual nanoparticles in real time — turning Brownian motion into size and absolute count, an applied field into zeta potential, and fluorescent labels into specificity. One instrument, six measurements, no calibration curves.
Nanoparticle tracking analysis follows particles one at a time rather than averaging a whole suspension. That single difference is what lets ZetaView resolve a polydisperse sample into its real distribution, count particles in absolute terms, and tell a true subpopulation apart from background.
Each panel below is a working model you can drive. Move a slider or switch a mode and the measurement responds the way the instrument does — so you can see exactly what changes when your sample, your label, or your settings change.
Laser illumination, a temperature-controlled measurement cell, and a sensitive sCMOS camera sit behind a single touchscreen — so size, concentration, zeta potential, and fluorescence all come from the same particles, in the same run.
Brownian motion is faster for small particles and slower for large ones. The Stokes–Einstein relation converts each tracked path into a hydrodynamic diameter, and thousands of particles build a full distribution — not a single average.
Drag polydispersity and switch sample type. Watch D10 / D50 / D90 and the span respond — a monodisperse standard collapses to a sharp peak; a real EV prep spreads wide.
Rather than reading one field of view, ZetaView scans eleven positions through the measurement cell and averages them. That spatial sampling delivers an absolute particle count across a wide dynamic range — and flags when a sample is too sparse to be reliable or so crowded that tracks overlap.
Slide the concentration. The optimal band gives a trustworthy count; go too low and counting statistics get noisy, too high and coincidence makes the instrument under-count until you dilute.
Apply an electric field and charged particles migrate; their velocity reveals the zeta potential — the effective surface charge that governs colloidal stability. ZetaView measures this in the same cell, on the same particles it just sized.
Sweep the pH. Near the isoelectric point the charge collapses toward zero and particles aggregate; push pH away and |ζ| climbs past 30 mV into the stable regime.
Light scatter sees every particle — vesicles, aggregates, and contaminants alike. Add a fluorescent label and ZetaView counts only what carries your marker, separating specific signal from a noisy background in the very same sample.
Toggle scatter and fluorescence, pick a tetraspanin channel, and set how many particles carry the marker. Specific count is always a subset of total scatter — that gap is your specificity.
Two labels, two channels. Plotting each particle by both intensities sorts the population into four quadrants — and the particles that light up in both are the colocalized, double-positive events that prove two markers share one vesicle.
Each dot is a particle. Drive the colocalization slider to move events into the double-positive quadrant — the population a bulk assay would never resolve.
Real preparations are mixtures — bright mature vesicles, dim immature ones, and free-protein debris all overlap. Gating on size and fluorescence intensity together isolates a genuine subpopulation and reports its count, fraction, and characteristic size.
Drag both gates to draw a box around a cluster. The instrument reports only what falls inside — letting you separate true EV subsets from background a single parameter would blur together.
The same multiparameter read supports academic discovery and regulated manufacturing — characterising biological nanoparticles wherever size, count, charge, and markers all matter.
Size, concentration, and tetraspanin profiling with per-EV fluorescence.
Size and zeta for LNP stability in mRNA and gene-delivery work.
Titre, integrity, and stability for diagnostics and vaccine development.
Lot-to-lot consistency and stability studies for GLP/GMP workflows.
An NTA platform used across peer-reviewed EV, virus, and nanomedicine literature.
Size, concentration, zeta, and fluorescence on the same particles — no instrument-hopping.
Scripted protocols and automated parameter control generate data that stands up in GLP/GMP settings.
For Research Use Only. Not for use in diagnostic procedures.