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Particle Metrix · Triple-fluorescence NTA

Three lasers, three markers, one particle at a time.

The ZetaView® x30 adds multi-laser fluorescence and an extended optical path to nanoparticle tracking — so you can distinguish CD9, CD63, and CD81 subpopulations on individual vesicles, even in complex or turbid samples, with data integrity built for regulated workflows.

3-laser fluorescence · 488 / 561 / 640 nmTriple
488 561 640 CD9 · CD63 · CD81
Per-vesicle multi-marker phenotypex30
3
Fluorescence channels
30–1000
Particle size range (nm)
21 CFR 11
Data-integrity ready
3 in 1
Size · count · zeta
What the x30 adds

Specificity, on heterogeneous samples.

Where the Evolution measures size, concentration, zeta, and single-channel fluorescence, the x30 extends the platform with multiple laser lines and a longer optical path. That combination is what lets it pull apart overlapping vesicle subtypes — the CD9-only, CD63-only, and co-labelled populations that a single channel blends together.

It is built for labs bridging discovery to manufacturing: the same instrument that resolves a research question also generates the audited, traceable records a release assay needs.

Signature capability

Triple-channel detection

Each particle in the field carries its own combination of surface markers. Switch laser channels on and off to reveal which subpopulations light up — and which vesicles are co-labelled across two or three markers at once.

Toggle channels. A particle gains a coloured ring for every active marker it carries; two or three rings means a genuinely co-labelled vesicle — the phenotype bulk assays average away.

CD9+
CD63+
CD81+
Multi-positive
Fluorescence field · per-particle markersx30
Active laser channels 3 of 3
Why ZetaView x30

From a research answer to a release record.

Specificity

Distinguish EV subtypes

Separate CD9 / CD63 / CD81 populations, VLPs, and engineered nanocarriers on individual particles.

Optics

Extended optical path

Higher resolution in complex or turbid samples where single-path systems struggle.

Compliance

21 CFR Part 11 ready

Electronic records and signatures with the data-integrity tools regulated submissions expect.

Real time

Size, count & zeta together

Multiparameter tracking in one workflow — no moving the sample between instruments.

Scale

Bench to bioprocess

Reproducible across sites, from a single research group to industrial bioproduction.

Submissions

FDA · EMA · PMDA

Data-integrity features that support regulatory filings across major agencies.

Where it fits

Built for translational and regulated work.

Advanced EV research

Differentiate heterogeneous EV populations by surface phenotype.

Gene therapy & mRNA

Characterise lipid nanoparticles for drug and gene delivery.

QC in bioproduction

Release testing for viral vectors and nanoparticle products.

Diagnostics development

Fluorescently labelled exosome and virus detection assays.

At a glance

ZetaView x30 specifications.

Modalities
Scatter & fluorescence NTA · electrophoretic zeta potential
Fluorescence
Three laser lines (e.g. 488 / 561 / 640 nm) for multi-marker detection
Size range
30 – 1000 nm, depending on particle refractive index and mode
Optics
Extended optical pathway for complex and turbid samples
Compliance
21 CFR Part 11 electronic records & signatures; data-integrity tooling
Scanning
Concentration Scanning Technology — multiple cell positions averaged
Interactive illustration is a teaching model of multi-channel behaviour — representative, not an instrument specification or a substitute for method validation.

Resolve the subpopulations your samples are hiding.

Request a demo

For Research Use Only. Not for use in diagnostic procedures.