Single-molecule sensitivity
Validated detection efficiency near 100% across more than 30 fluorescent dyes. Every photon-emitting molecule is counted — including the dimmest — not estimated from a curve.
The Pangnostics dFC is an ultra-high-sensitivity single-molecule counter in a familiar flow-cytometry format — counting, sizing, and phenotyping every extracellular vesicle, LNP, viral vector, and antibody that flows through, without a single calibration bead.
For decades, flow cytometry has driven our understanding of cellular heterogeneity. But when the same instruments are turned on the nanoscale — extracellular vesicles, lipid nanoparticles, viral vectors — a large fraction of every sample stays hidden below the instrument's detection floor. Most EVs are smaller than 70 nm and dimmer than a handful of fluorophores, so they simply aren't counted. Every downstream conclusion inherits that blind spot.
Pangnostics dFC closes the gap with a fundamentally different optical design: planar microfluidics and line-confocal detection that count individual fluorophore-tagged molecules directly, with essentially 100% single-molecule detection efficiency. The result is absolute, calibration-free quantitation — and a per-particle view of size, surface markers, and copy number that bulk methods average away.
Four steps turn a drop of sample into an absolute, per-particle measurement. Step through each one.
Because the smallest vesicles dominate most preparations, where a method's detection floor sits determines both how many EVs you count and how large they appear. Drag the floor from single-molecule sensitivity (≤35 nm) toward conventional limits and watch the captured fraction collapse while the apparent median size climbs.
Bulk methods report an average: "this prep carries marker X." dFC counts how many copies sit on each individual vesicle and builds the distribution. Two preparations can share an identical bulk average yet be completely different biologies — many lightly-decorated EVs, or a few heavily-loaded ones. Drag to hold the bulk number fixed and watch the per-particle truth change.
Validated detection efficiency near 100% across more than 30 fluorescent dyes. Every photon-emitting molecule is counted — including the dimmest — not estimated from a curve.
Four lasers and twelve simultaneous channels resolve surface-marker combinations on individual nanoparticles in a single run — expandable for higher-plex assays.
Direct digital counting yields true concentrations and biomarker copy numbers — no calibration beads, no reference ladders, no ~20% bead-calibration uncertainty.
Run a single urgent sample or a full 96-well plate on the same platform, with a familiar flow-cytometry workflow and no specialist operator required.
Surfaces the dim, small, and rare subpopulations — well under 1% of total particles — that bulk and commercial high-sensitivity platforms miss by design.
Measures the absolute concentration of antibody-dye conjugates that can't be read at 280 nm, plus dye-to-protein ratio — removing lot-to-lot reagent variability as a source of irreproducibility.
The same workflow and the same rigor across the biological nanoparticles that matter to therapeutic and diagnostic programs.
Phenotype individual EVs across twelve colors; quantify tetraspanin and engineered-marker copy number and absolute subpopulation concentration for liquid biopsy and EV therapeutics.
Characterize mRNA and gene-therapy LNPs at the single-particle level — payload distribution, surface composition, and encapsulation uniformity.
Distinguish full, empty, and partial capsids; measure titer and payload heterogeneity for vector development and release characterization.
Resolve oligomeric states and RNA-binding protein complexes that bulk methods cannot separate from vesicular populations.
Authenticate dye-to-protein ratios, quantify aggregation, and verify conjugate concentration — removing reagent variability from your workflow.
Reference-grade absolute concentrations make dFC an ideal orthogonal method for cross-laboratory harmonization and reagent QC.
Absolute counting removes the calibration drift, curve-fitting, and vendor-dependent reagent variability that compromise quantitative EV flow cytometry. Copy-number and sizing methods have been validated against orthogonal techniques including super-resolution and single-molecule TIRF microscopy, and the platform is designed to support standardized community reporting.
For Research Use Only. Not for use in diagnostic procedures.